Born in Mumbai into a Mangalorean Catholic family, D'Souza was raised a Roman Catholic in the Bandra suburb of Mumbai. Her mother Jeanette D'Souza was formerly a secretary to managing director with a Pharma Multinational corporation (MNC), who left her job in 2004 to help Genelia with her career. Her father Neil D'Souza, is a senior official with Tata Consultancy Services (TCS). She also has a younger brother, Nigel D'Souza, who works with the Bombay Stock Exchange.
